CD57 Antibody [SPM129]

ProSci
Product Code: PSI-33-369
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-369-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgM, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM129
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Formalin-fixed, paraffin-embedded human spleen stained with CD57 antibody (SPM129).

Formalin-fixed, paraffin-embedded human spleen stained with CD57 antibody (SPM129).

Further Information

Additional Names:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P, B3GAT1, GLCATP
Application Note:
Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the CD57 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human peripheral blood mononuclear cells were used as the immunogen for the CD57 antibody.
NCBI Gene ID #:
27087
NCBI Official Name:
beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
NCBI Official Symbol:
B3GAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
PEG precipitation
Research Area:
Immunology
SPECIFICITY:
Does not react with rat
Swissprot #:
Q9P2W7
User NOte:
Optimal dilutions for each application to be determined by the researcher