Bax Antibody [6A7]

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ProSci
Product Code: PSI-99-623
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-99-623-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: [6A7]
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Monkey
  • Mouse
  • Rat
Application: Flow Cytometry
Shipping:
blue ice
Storage:
Store vial at 2-8˚C

Images

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Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bax-UNLB (Cat. No. 99-623). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection.
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Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bax-UNLB (Cat. No. 99-623). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection.

Documents

Further Information

Additional Names:
Apoptosis regulator BAX, BCL2 associated X protein
Application Note:
Bax Antibody [6A7] for use in flow cytometry, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, immunoprecipitation, and neutralization assays.
Background:
Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes, it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however, treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax but not Bax complexed with either Bcl-2 or Bcl-xL.
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
0.1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Bax peptide (a.a. 12-24) common to mouse, rat, and human
NCBI Gene ID #:
581/12028/24887
NCBI Official Name:
BCL2 associated X, apoptosis regulator
NCBI Official Symbol:
BAX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q07812
Research Area:
Cancer,Cell Cycle,Obesity
SPECIFICITY:
Bax
Swissprot #:
Q07812
User NOte:
Optimal dilutions for each application to be determined by the researcher.