Beta Catenin Antibody [6F9]

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ProSci
Product Code: PSI-33-622
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-622-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 6F9
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Chicken (Gallus)
  • Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human tonsil tissue with b-Catenin antibody (clone 6F9). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
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IHC testing of FFPE human tonsil tissue with b-Catenin antibody (clone 6F9). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

Documents

Further Information

Additional Names:
Catenin beta-1, Beta-catenin, CTNNB1, CTNNB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

WB: 0.5-1 ug/ml

IHC (FFPE): 1-2 ug/ml

Optimal dilution of the b-Catenin antibody to be determined by the researcher.
Background:
Beta Catenin is a dual function protein, regulating the coordination of cell?cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. b-Catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-Catenin, also known as plakoglobin. b-Catenin is widely expressed in many tissues. In cardiac muscle, it localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocyte. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chicken beta-Catenin was used as the immunogen for the b-Catenin antibody.
NCBI Gene ID #:
1499
NCBI Official Name:
catenin (cadherin-associated protein), beta 1, 88kDa
NCBI Official Symbol:
CTNNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P35222
User NOte:
Optimal dilutions for each application to be determined by the researcher