Beta Catenin Antibody [CTNNB1/1508]

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ProSci
Product Code: PSI-33-616
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-616-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CTNNB1/1508
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human pancreas tissue with Beta Catenin antibody (clone CTNNB1/1508). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
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IHC testing of FFPE rat colon tissue with Beta Catenin antibody (clone CTNNB1/1508). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
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IHC testing of FFPE human pancreas tissue with Beta Catenin antibody (clone CTNNB1/1508). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
IHC testing of FFPE rat colon tissue with Beta Catenin antibody (clone CTNNB1/1508). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

Documents

Further Information

Additional Names:
Catenin beta-1, Beta-catenin, CTNNB1, CTNNB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

WB: 0.5-1 ug/ml

IHC (FFPE): 1-2 ug/ml

Optimal dilution of the Beta Catenin antibody to be determined by the researcher.
Background:
Beta-Catenin associates with the cytoplasmic portion of E-Cadherin, which is necessary for the function of E-Cadherin as an adhesion molecule. In normal tissues, beta-Catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-Catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immunostaining of beta-Catenin and E-Cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A partial human recombinant protein was used as the immunogen for the Beta Catenin antibody.
NCBI Gene ID #:
1499
NCBI Official Name:
catenin (cadherin-associated protein), beta 1, 88kDa
NCBI Official Symbol:
CTNNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P35222
User NOte:
Optimal dilutions for each application to be determined by the researcher