E-Cadherin Antibody [ECD1-2]

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ProSci
Product Code: PSI-34-050
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-34-050-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: ECD1-2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human skin with E-Cadherin antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
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IHC testing of FFPE human skin with E-Cadherin antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.

Documents

Further Information

Additional Names:
Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO
Application Note:
Western blot: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT

Immunofluorescence: 0.5-1 ug/ml

Optimal dilution of the E-Cadherin antibody should be determined by the researcher.
Background:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
NCBI Gene ID #:
999
NCBI Official Name:
cadherin 1, type 1, E-cadherin (epithelial)
NCBI Official Symbol:
CDH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle,Signal Transduction
SPECIFICITY:
Not suitable for Mouse, Rat sample testing
Swissprot #:
P12830
User NOte:
Optimal dilutions for each application to be determined by the researcher