Recombinant Caldesmon Antibody [CALD1/1424R]

ProSci
Product Code: PSI-34-070
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-34-070-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG, k
Antibody Clonality: Recombinant Antibody
Antibody Clone: CALD1/1424R
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human uterus with recombinant Caldesmon antibody (clone CALD1/1424R). Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min.
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IHC testing of FFPE human uterus with recombinant Caldesmon antibody (clone CALD1/1424R). Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min.

Further Information

Additional Names:
Caldesmon, CDM, CALD1, CAD, CDM
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the recombinant Caldesmon antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Caldesmon (CAD, CALD, CALD1) is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A full length human protein was used as the immunogen for the recombinant Caldesmon antibody.
NCBI Gene ID #:
800
NCBI Official Name:
caldesmon 1
NCBI Official Symbol:
CALD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
Q05682
User NOte:
Optimal dilutions for each application to be determined by the researcher