Recombinant Calponin Antibody [CNN1/1408R]

ProSci
Product Code: PSI-34-066
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-34-066-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG, k
Antibody Clonality: Recombinant Antibody
Antibody Clone: CNN1/1408R
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

1 / 2
IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
2 / 2

IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

Further Information

Additional Names:
CNN1, Basic calponin, Calponin, Calponin-1, Calponins, basic, Calponin H1, smooth muscle, SMCC, Sm-Calp
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant Calponin antibody.
NCBI Gene ID #:
1264
NCBI Official Name:
calponin 1, basic, smooth muscle
NCBI Official Symbol:
CNN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Signal Transduction, Stem Cell
Swissprot #:
P51911
User NOte:
Optimal dilutions for each application to be determined by the researcher