Calponin Antibody [CLPN5-1]

ProSci
Product Code: PSI-33-902
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-902-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CLPN5-1
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human uterus and Calponin antibody (clone CLPN5-1).
2 / 2

IHC testing of FFPE human uterus and Calponin antibody (clone CLPN5-1).

Further Information

Additional Names:
CNN1, Basic calponin, Calponin, Calponin-1, Calponins, basic, Calponin H1, smooth muscle, SMCC, Sm-Calp
Application Note:
Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the Calponin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein was used as the immunogen for the Calponin antibody.
NCBI Gene ID #:
1264
NCBI Official Name:
calponin 1, basic, smooth muscle
NCBI Official Symbol:
CNN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P51911
User NOte:
Optimal dilutions for each application to be determined by the researcher