Caspase-3 (Pro and Active) Antibody [CAS3836]

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ProSci
Product Code: PSI-33-007
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-007-100ug100ug£662.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CAS3836
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Western blot testing of staurosporine-treated Jurkat cells (2 hr, 2 uM) with Caspase-3 antibody at 2ug/ml. The pro form is seen at ~32kD and active caspase-3 seen at ~17kD and ~12kD.
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Western blot testing of staurosporine-treated Jurkat cells (2 hr, 2 uM) with Caspase-3 antibody at 2ug/ml. The pro form is seen at ~32kD and active caspase-3 seen at ~17kD and ~12kD.

Documents

Further Information

Additional Names:
Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3
Application Note:
Western blot: 2-4 ug/ml

This antibody will detect the pro form and p17 and p12 active forms of Caspase-3.
Background:
Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12). Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.5 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The large subunit domain of human Caspase-3 was used as the immunogen for this monoclonal antibody.
NCBI Gene ID #:
836
NCBI Official Name:
caspase 3, apoptosis-related cysteine peptidase
NCBI Official Symbol:
CASP3
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G purified monoclonal antibody
Research Area:
Apoptosis
Swissprot #:
P42574
User NOte:
Optimal dilutions for each application to be determined by the researcher