Recombinant CD20 Antibody [IGEL/1497R]

ProSci
Product Code: PSI-34-058
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-34-058-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: IGEL/1497R
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. Required HIER: steam section in pH6 citrate buffer for 20 min.

IHC testing of recombinant CD20 antibody and FFPE human tonsil tissue. Required HIER: steam section in pH6 citrate buffer for 20 min.

Further Information

Additional Names:
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the recombinant CD20 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Full length human CD20 protein was used as the immunogen for this reccombinant CD20 antibody.
NCBI Gene ID #:
931
NCBI Official Name:
membrane-spanning 4-domains, subfamily A, member 1
NCBI Official Symbol:
MS4A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P11836
User NOte:
Optimal dilutions for each application to be determined by the researcher