B7-H3 Antibody [10G6]

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ProSci
Product Code: PSI-RF16095
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-RF16095-0.02mg0.02mg£150.00
Quantity:
PSI-RF16095-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1,k
Antibody Clonality: Monoclonal
Antibody Clone: 10G6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
B7-H3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
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Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.<br><br>Green: B7-H3 Antibody [10G6] (RF16095) <br> Blue: DAPI staining
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Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml.<br><br>Green: B7-H3 Antibody [10G6] (RF16095) <br> Blue: DAPI staining
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Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.
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Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
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Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with RF16091, RF16092, RF16093, RF16094, and RF16095 antibodies at decreasing concentrations.
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Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.<br><br>Green: B7-H3 Antibody [10G6] (RF16095) <br> Blue: DAPI staining
Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml.<br><br>Green: B7-H3 Antibody [10G6] (RF16095) <br> Blue: DAPI staining
Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.
Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with RF16091, RF16092, RF16093, RF16094, and RF16095 antibodies at decreasing concentrations.

Documents

Further Information

Additional Names:
B7-H3 Antibody: CD276 molecule, B7H3, B7RP-2, 4Ig-B7-H3, CD276
Application Note:
B7-H3 antibody can be used for detection of B7-H3 for immunohistochemistry starting at 2 μg/mL and Immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 1 μg/ml.

Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.
Background:
CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
Background References:
  • Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2001; 2:269-74.
  • Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes. J Immunol 2002; 168:6294-7.
  • Yi KH and Chen L. Fine tuning the immune response through B7-H3 and B7-H4. Immunol Rev 2009; 229:145-51.
  • Wang L, Kang FB, and Shan BE. B7-H3-mediated tumor immunology: friend or foe. Int J Cancer 2014; 134:2764-71.
Buffer:
B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
B7-H3 antibody was raised against the extracellular domain of human B7-H3
NCBI Gene ID #:
80381
NCBI Official Name:
CD276
NCBI Official Symbol:
CD276
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001019907
Protein GI Number:
67188443
Purification:
B7-H3 Antibody is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
Q5ZPR3
User NOte:
Optimal dilutions for each application to be determined by the researcher.