CD3 Antibody [CT-3]

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ProSci
Product Code: PSI-99-201
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-99-201-0.5mg0.5mg£597.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: [CT-3]
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Pigeon
Application: Immunohistochemistry- Frozen Section (IHC-F)
Shipping:
blue ice
Storage:
Store vial at 2-8˚C

Images

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Paraffin embedded chicken spleen tissue was stained with Mouse Anti-Chicken CD3-UNLB (Cat. No. 99-201; right) followed by Anti-Mouse IgG-HRP and DAB.
2 / 3
Frozen vIBDV infected bursal tissue section was stained with Mouse Anti-Chicken CD3-UNLB (Cat. No. 99-201) followed by a secondary reagents and DAB. Image from Rauf A, Khatri M, Murgia MV, Jung K, Saif YM. Differential modulation of cytokine, chemokine and Toll like receptor expression in chickens infected with classical and variant infectious bursal disease virus. Vet Res. 2011;42:85. Figure 3[A(e)]
3 / 3
Frozen chicken ovary section was stained with Mouse Anti-Chicken CD3-UNLB (Cat. No. 99-201) followed by an HRP conjugated secondary antibody, DAB, and hematoxylin. Image from Bradaric MJ, Barua A, Penumatsa K, Yi Y, Edassery SL, Sharma S, et al. Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors. J Ovarian Res. 2011;4:4. Figure 6(b)
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Paraffin embedded chicken spleen tissue was stained with Mouse Anti-Chicken CD3-UNLB (Cat. No. 99-201; right) followed by Anti-Mouse IgG-HRP and DAB.
Frozen vIBDV infected bursal tissue section was stained with Mouse Anti-Chicken CD3-UNLB (Cat. No. 99-201) followed by a secondary reagents and DAB. Image from Rauf A, Khatri M, Murgia MV, Jung K, Saif YM. Differential modulation of cytokine, chemokine and Toll like receptor expression in chickens infected with classical and variant infectious bursal disease virus. Vet Res. 2011;42:85. Figure 3[A(e)]
Frozen chicken ovary section was stained with Mouse Anti-Chicken CD3-UNLB (Cat. No. 99-201) followed by an HRP conjugated secondary antibody, DAB, and hematoxylin. Image from Bradaric MJ, Barua A, Penumatsa K, Yi Y, Edassery SL, Sharma S, et al. Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors. J Ovarian Res. 2011;4:4. Figure 6(b)

Documents

Further Information

Additional Names:
T3/TCR complex
Application Note:
CD3 Antibody [CT-3] for use in flow cytometry, immunohistochemistry, immunoprecipitation, and stimulation assays.
Background:
CD3 is a member of the T cell receptor-associated CD3 complex. The monoclonal antibody CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa.
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
0.5 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chicken thymocytes and Ig- blood mononuclear cells
NCBI Gene ID #:
396518
NCBI Official Name:
CD3d molecule
NCBI Official Symbol:
CD3D
NCBI Organism:
Gallus gallus
Physical State:
Liquid
Research Area:
Immunology
SPECIFICITY:
CD3
User NOte:
Optimal dilutions for each application to be determined by the researcher.