Recombinant CD45RB Antibody [PTPRC/1783R]
| Code | Size | Price |
|---|
| PSI-33-819-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG, k
Antibody Clonality: Recombinant Antibody
Antibody Clone: PTPRC/1783R
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistology (FFPE): 0.5-1 ug/ml for 30 minutes at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Western blot: 0.5-1 ug/ml
Due to variation in protocol and secondary antibody used, the recombinant CD45RB antibody may need to be titered for optimal performance.
Immunofluorescence: 0.5-1 ug/ml
Immunohistology (FFPE): 0.5-1 ug/ml for 30 minutes at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Western blot: 0.5-1 ug/ml
Due to variation in protocol and secondary antibody used, the recombinant CD45RB antibody may need to be titered for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human B lymphocytes were used as immunogen for this recombinant CD45RB antibody.
NCBI Gene ID #:
5788
NCBI Official Name:
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol:
PTPRC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Cancer,Immunology,Neuroscience,Signal Transduction
Swissprot #:
P08575
User NOte:
Optimal dilutions for each application to be determined by the researcher