CD45RA Antibody [CDLA45RA-1]

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45

Flow Cytometry: 0.5-1 ug/million cells

Immunofluorescence: 1-2 ug/ml

Immunohistology (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the anti-CD45RA antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Stimulated human leukocytes were used as immunogen for this anti-CD45RA antibody.

5788

protein tyrosine phosphatase, receptor type, C

PTPRC

Homo sapiens

Liquid

Protein G affinity chromatography

Cancer,Immunology,Neuroscience,Signal Transduction

P08575

Optimal dilutions for each application to be determined by the researcher