CD74 Antibody [CDLA74-1]
| Code | Size | Price |
|---|
| PSI-34-016-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CDLA74-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
CD74, DHLAG, HLADG, Ia-GAMMA, CD74 molecule, major histocompatibility complex, class II invariant chain, CD74 antigen, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), HLA-DR-gamma, Ia-associated invar
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry: 1-2 ug/ml (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the CD74 antibody may be required for optimal performance.
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry: 1-2 ug/ml (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the CD74 antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. The CD74 antibody epitope is known to be extracellular.
Background:
CD74 associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [RefSeq]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant full-length human protein was used as the immunogen for the CD74 antibody. (3)
NCBI Gene ID #:
972
NCBI Official Name:
CD74 molecule, major histocompatibility complex, class II invariant chain
NCBI Official Symbol:
CD74
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
P04233
User NOte:
Optimal dilutions for each application to be determined by the researcher