CD99 Antibody

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ProSci
Product Code: PSI-33-638
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-638-100ug100ug£765.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human Ewing's sarcoma with CD99 antibody. Required HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
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IHC testing of FFPE human Ewing's sarcoma with CD99 antibody. Required HIER: steam sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Documents

Further Information

Additional Names:
CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 molecule, E2 antigen, HBA71
Application Note:
Flow Cytometry: 2-4 ug/million cells in 0.1ml

Immunofluorescence: 2-4 ug/ml

Immunohistochemistry (FFPE): 5-10 ug/ml for 30 min at RT

Titering of the CD99 antibody may be required for optimal performance.
Background:
CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein was used as the immunogen for the CD99 antibody.
NCBI Gene ID #:
4267
NCBI Official Name:
CD99 molecule
NCBI Official Symbol:
CD99
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P14209
User NOte:
Optimal dilutions for each application to be determined by the researcher