CD28 Antibody [AV7] (Biotin)
| Code | Size | Price |
|---|
| PSI-99-238-0.5mg | 0.5mg | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: [AV7]
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Turkey
Applications:
- Blocking
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunoprecipitation (IP)
Shipping:
blue ice
Storage:
Store vial at 2-8˚C
Documents
Further Information
Application Note:
CD28 Antibody [AV7] for use in flow cytometry, immunohistochemistry / immunocytochemistry, and immunoprecipitation assays.
Background:
Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on antigen presenting cells. Peripheral gammadelta+ T cells are CD28 negative. Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A, or monoclonal antibodies against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A but inhibited by herbimycin A suggesting that CD28 employs a signaling pathway at least partly distinct from that triggered by TCR/CD3. The monoclonal antibody AV7 also reacts with turkey CD28 as demonstrated by flow cytometry.
Buffer:
Supplied in PBS/NaN3
Concentration:
0.5 mg/mL
Conjugate:
Biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chicken peripheral blood lymphocytes
NCBI Gene ID #:
396249
NCBI Official Name:
CD28 molecule
NCBI Official Symbol:
CD28
NCBI Organism:
Gallus gallus
Physical State:
Liquid
Protein Accession #:
P31043
Research Area:
Immunology,Signal Transduction
SPECIFICITY:
CD28
Swissprot #:
P31043
User NOte:
Optimal dilutions for each application to be determined by the researcher.