CD45 Antibody [LT40] (Biotin)
| Code | Size | Price |
|---|
| PSI-99-242-0.5mg | 0.5mg | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgM
Antibody Clonality: Monoclonal
Antibody Clone: [LT40]
Regulatory Status: RUO
Target Species: Chicken (Gallus)
Application: Immunocytochemistry (ICC)
Shipping:
blue ice
Storage:
Store vial at 2-8˚C
Documents
Further Information
Application Note:
CD45 Antibody [LT40] for use in flow cytometry, immunohistochemistry, immunoprecipitation, and separation assays.
Background:
Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45. Levels of CD45 expression detected by the monoclonal antibody LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45low) of thymocytes. The CD45low population contains exclusively thymocytes with the CD3-CD4-CD8low phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8low to CD4+CD8+ and before the expression of CD3. The ability of the monoclonal antibody LT40 to discriminate this CD45low subpopulation provides a suitable marker for the CD3-CD4-CD8low immediate precursors to the CD3-CD4+CD8+ thymocytes.
Buffer:
Supplied in PBS/NaN3
Concentration:
0.5 mg/mL
Conjugate:
Biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chicken bursal lymphocytes
NCBI Gene ID #:
386580
NCBI Official Name:
protein tyrosine phosphatase, receptor type C
NCBI Official Symbol:
PTPRC
NCBI Organism:
Gallus gallus
Physical State:
Liquid
Protein Accession #:
Q9I8D7
Research Area:
Immunology,Neuroscience,Signal Transduction,Stem Cell
SPECIFICITY:
CD45
Swissprot #:
Q9I8D7
User NOte:
Optimal dilutions for each application to be determined by the researcher.