Golgi Complex Antibody [371-4]
| Code | Size | Price |
|---|
| PSI-33-246-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 371-4
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Application Note:
Western Blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunocytochemistry: (Acetone/paraformaldehyde fixed) 0.5-1 ug/ml for 30 min
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Golgi complex antibody to be titered up or down for optimal performance.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunocytochemistry: (Acetone/paraformaldehyde fixed) 0.5-1 ug/ml for 30 min
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Golgi complex antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
Clone 371-4 antibody specifically detects the Golgi complex in human cells. It is part of panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. The antibody recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a diffuse staining pattern of the Golgi zone in normal and malignant cells and may be used to stain Golgi complex in frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. The 371-4 antibody is an excellent marker for human cells in xenographic model research.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SU-DHL-1 large cell lymphoma cells were used as the immunogen.
NCBI Gene ID #:
Unknown
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Obesity, Signal Transduction
User NOte:
Optimal dilutions for each application to be determined by the researcher