Cytokeratin 17 Antibody [CTKN17-1]

ProSci
Product Code: PSI-33-965
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-965-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CTKN17-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE cervical carcinoma and Cytokeratin 17 antibody.
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IHC testing of FFPE cervical carcinoma and Cytokeratin 17 antibody.

Further Information

Additional Names:
KRT17, K17, PC, PC2, PCHC1
Application Note:
Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the Cytokeratin 17 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Cytokeratin 17 is a type I keratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant full-length human KRT17 protein was used as the immunogen for the Cytokeratin 17 antibody.
NCBI Gene ID #:
3872
NCBI Official Name:
keratin 17
NCBI Official Symbol:
KRT17
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
Q04695
User NOte:
Optimal dilutions for each application to be determined by the researcher