dsDNA Antibody [DRNC1-1]
Code | Size | Price |
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PSI-34-014-100ug | 100ug | £765.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: IgG3, kappa
Antibody Clonality: Monoclonal
Antibody Clone: DRNC1-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Images
Documents
Further Information
Application Note:
Immunofluorescence: 1-2 ug/ml
Immunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RT
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
Titering of the dsDNA antibody may be required for optimal performance.
Immunocytochemistry (Acetone-fixed): 0.5-1 ug/ml for 30 min at RT
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
Titering of the dsDNA antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
Deoxyribonucleic acid (DNA) is a nucleic acid that stores long-term information regarding the development and function of all known living organisms. DNA consists of two long nucleotide polymers, which are composed of four bases, namely adenine, thymine, guanine and cytosine, all of which are flanked by a phosphate-deoxyribose backbone. This mAb can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Cell nuclei were used as the immunogen for the dsDNA antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
Not Known
User NOte:
Optimal dilutions for each application to be determined by the researcher