DOG1 Antibody [DG1/1485]

ProSci
Product Code: PSI-33-697
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-697-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: DG1/1485
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (DG1/1485). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (DG1/1485). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Further Information

Additional Names:
TMEM16A Antibody: DOG1, TAOS2, ORAOV2, TMEM16A, DOG1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

IF: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.
Background:
DOG1 is expressed in the human gastrointestinal epithelium and is highly expressed in the gastrointestinal interstitial cells of Cajal, where it plays an important role in epithelial chloride secretion mediating intestinal motility. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.
NCBI Gene ID #:
55107
NCBI Official Name:
anoctamin 1, calcium activated chloride channel
NCBI Official Symbol:
ANO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
Q5XXA6
User NOte:
Optimal dilutions for each application to be determined by the researcher