ICAD Antibody
| Code | Size | Price |
|---|
| PSI-33-028-100ug | 100ug | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit
Application Note:
Western blot: 4-8 ug/ml
Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
Background:
During apoptosis, toxic or useless cells are broken down for removal. DFF, or DNA fragmentation factor, is a heterodimeric protein which mediates DNA fragmentation and chromatin condensation during apoptosis. DFFB is the active DFF subunit but remains inactive while bound to DFFA, also call ICAD (Inhibitor of caspase-activated DNase). Caspase-3 cleaves ICAD, which then disassociates from DFFB. The disassociation removes the inhibitory effects of ICAD, activating DFFB and beginning the DNA fragmentation process.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.5 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant protein fragment from human ICAD was used as the immunogen for this antibody.
NCBI Gene ID #:
1676
NCBI Official Name:
DNA fragmentation factor, 45kDa, alpha polypeptide
NCBI Official Symbol:
DFFA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Apoptosis
Swissprot #:
O00273
User NOte:
Optimal dilutions for each application to be determined by the researcher