EpCAM Antibody

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ProSci
Product Code: PSI-33-679
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-679-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human renal cell carcinoma and EpCAM antibody. Required HIER: steam sections in pH6 citrate buffer for 10-20 min.
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IHC testing of FFPE human renal cell carcinoma and EpCAM antibody. Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

Documents

Further Information

Additional Names:
Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Application Note:
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT

Titering of the EpCAM antibody may be required for optimal performance.
Background:
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human recombinant protein was used as the immunogen for the EpCAM antibody.
NCBI Gene ID #:
4072
NCBI Official Name:
epithelial cell adhesion molecule
NCBI Official Symbol:
EPCAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle,Signal Transduction
SPECIFICITY:
Does not react with mouse or rat
Swissprot #:
P16422
User NOte:
Optimal dilutions for each application to be determined by the researcher