CELA3B Antibody [CELA3B/1257]

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ProSci
Product Code: PSI-33-782
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-782-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: CELA3B/1257
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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SDS-PAGE Analysis of Purified, BSA-Free Elastase 3B Antibody (clone CELA3B/1257). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Elastase 3B Antibody (clone CELA3B/1257). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B
Application Note:
ELISA: 2-4 ug/ml; order BSA free format for coating

Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 1-2 ug/ml

IHC-P: 1-2 ug/ml for 30 min at RT

Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires

Background:
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.
NCBI Gene ID #:
23436
NCBI Official Name:
Chymotrypsin-like elastase family member 3B
NCBI Official Symbol:
CELA3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P08861
User NOte:
Optimal dilutions for each application to be determined by the researcher