Erythropoietin Antibody [EPO/1368]
| Code | Size | Price |
|---|
| PSI-33-731-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: EPO/1368
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Erythropoietin, Epoetin, EPO
Application Note:
WB: 0.5-1 ug/ml
IF: 1-2 ug/ml
Immunohistochemistry (FFPE): 4-8 ug/ml for 30 min at RT (1)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Titering of the EPO antibody may be required for optimal performance.
IF: 1-2 ug/ml
Immunohistochemistry (FFPE): 4-8 ug/ml for 30 min at RT (1)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Titering of the EPO antibody may be required for optimal performance.
1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Background:
Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein was used as the immunogen for the EPO antibody.
NCBI Gene ID #:
2056
NCBI Official Name:
erythropoietin
NCBI Official Symbol:
EPO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P01588
User NOte:
Optimal dilutions for each application to be determined by the researcher