ER Antibody [ER506]

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ProSci
Product Code: PSI-33-127
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-127-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: ER506
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of human breast invasive ductal carcinoma with ER antibody (ER506).
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Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~66kDa.
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IHC testing of human breast invasive ductal carcinoma with ER antibody (ER506).
Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~66kDa.

Documents

Further Information

Additional Names:
ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER
Application Note:
Western blot: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells

IHC (Formalin-paraffin): 1-2 ug/ml (1)

Variations in protocols, secondaries and substrates may require the ER antibody to be titered for optimal performance.

1. FFPE testing requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min and cooling at RT for 20 minutes.

Background:
Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. Clone ER506 antibody strongly stains nuclei of epithelial cells in breast carcinomas.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human ER alpha protein (aa 2-185) was used as the immunogen.
NCBI Gene ID #:
2099
NCBI Official Name:
estrogen receptor 1
NCBI Official Symbol:
ESR1
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P03372
User NOte:
Optimal dilutions for each application to be determined by the researcher