Coagulation factor XIII A chain Antibody [F13A1/1448]

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ProSci
Product Code: PSI-33-843
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-843-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: F13A1/1448
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Factor XIIIa antibody (clone F13A1/1448). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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SDS-PAGE Analysis of Purified, BSA-Free Factor XIIIa Antibody (clone F13A1/1448). Confirmation of Integrity and Purity of the Antibody.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Factor XIIIa antibody (clone F13A1/1448). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE Analysis of Purified, BSA-Free Factor XIIIa Antibody (clone F13A1/1448). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
F13A1, coagulation factor XIII, A1 polypeptide, RP11-232H4.1, F13A, Coagulation factor XIII A chain, Coagulation factor XIII, A polypeptide, TGase, bA525O21.1 (coagulation factor XIII, A1 polypeptide), coagulation factor XIII A1 subunit, factor XIIIa
Application Note:
ELISA: 2-4 ug/ml; order the BSA-free formulation for coating

Immunofluorescence: 1-2 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Western blot: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the Factor XIIIa antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Factor XIIIa has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-Factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein corresponding to amino acids 46-181 was used as the immunogen for the Factor XIIIa antibody.
NCBI Gene ID #:
2162
NCBI Official Name:
coagulation factor XIII, A1 polypeptide
NCBI Official Symbol:
F13A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P00488
User NOte:
Optimal dilutions for each application to be determined by the researcher