Fibronectin Antibody [SPM539]

ProSci
Product Code: PSI-34-109
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-34-109-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM539
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma stained with Fibronectin antibody (SPM539).

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma stained with Fibronectin antibody (SPM539).

Further Information

Additional Names:
FN1, CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, FINC, FN, LETS, MSF, FNZ, ED-B, GFND, GFND2
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

Background:
Fibronectin is a dimeric glycoprotein of 440kDa which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This mAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Purified human fibronectin was used as the immunogen for this Fibronectin antibody.
NCBI Gene ID #:
2335
NCBI Official Name:
fibronectin 1
NCBI Official Symbol:
FN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P02751
User NOte:
Optimal dilutions for each application to be determined by the researcher