Fibronectin Antibody [TV-1]

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ProSci
Product Code: PSI-33-136
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-136-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TV-1
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Porcine (pig)
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE pancreatic adenocarcinoma and Fibronectin antibody
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IHC testing of FFPE pancreatic adenocarcinoma and Fibronectin antibody

Documents

Further Information

Additional Names:
FN1, CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, FINC, FN, LETS, MSF, FNZ, ED-B, GFND, GFND2
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Fibronectin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Background:
Fibronectin is a soluble dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. There are two types of fibronectin: soluble fibronectin which is produced by hepatocytes and found in blood plasma, and insoluble cellular fibronectin which is produced by fibroblasts in a soluble form and latter assembled into an insoluble form via fibril formation.

This antibody reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this antibody localizes to tumor vessels where it binds to the underlying basement. The Fibronectin epitope recognized by this antibody is not accessible in normal tissues to the circulating antibody indicating that it can be used to specifically target tumor vessels in vivo. Clone TV-1 fibronectin antibody is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or antibodies.

Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A T-cell lymphoma biopsy was used as the immunogen for this fibronectin antibody.
NCBI Gene ID #:
2335
NCBI Official Name:
fibronectin 1
NCBI Official Symbol:
FN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P02751
User NOte:
Optimal dilutions for each application to be determined by the researcher