Filaggrin Antibody [FLG/1561]

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ProSci
Product Code: PSI-33-694
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-694-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: FLG/1561
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human skin with Filaggrin antibody (clone FLG/1561). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human skin with Filaggrin antibody (clone FLG/1561). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Documents

Further Information

Additional Names:
FLI1 Antibody : ATOD2, Filaggrin
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 1-2 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

Optimal dilution of the Filaggrin antibody should be determined by the researcher.
Background:
Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 998-1104 of human FLG were used as the immunogen for this Filaggrin antibody.
NCBI Gene ID #:
2312
NCBI Official Name:
Friend leukemia virus integration 1
NCBI Official Symbol:
FLI1
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Stem Cell
Swissprot #:
P20930
User NOte:
Optimal dilutions for each application to be determined by the researcher