FOXA1 Antibody [FOXA1/1512]
| Code | Size | Price |
|---|
| PSI-33-659-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: FOXA1/1512
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nuclear factor 3-alpha, Forkhead box protein A1, HNF-3-alpha
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the FOXA1 antibody should be determined by the researcher.
IF: 1-2 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the FOXA1 antibody should be determined by the researcher.
Background:
FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin as a pioneer factor. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
FOXA1 in breast cancer is highly correlated with ERalpha+, GATA3+, and PR+ protein expression as well as endocrine signaling. FOXA1 acts as a pioneer factor for ERa in ERalpha+ breast cancer, and its expression might identify ERalpha+ cancers that undergo rapid reprogramming of ERa signaling that is associated with poor outcomes and treatment resistance. Conversely, in ERalpha− breast cancer FOXA1 is highly correlated with low-grade morphology and improved disease free survival. FOXA1 is a downstream target of GATA3 in the mammary gland. Expression in ERalpha− cancers may identify a subset of tumors that is responsive to other endocrine therapies such as androgen receptor antagonist treatment. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
NCBI Gene ID #:
3169
NCBI Official Name:
forkhead box A1
NCBI Official Symbol:
FOXA1
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Stem Cell
SPECIFICITY:
Does not react with rat
Swissprot #:
P55317
User NOte:
Optimal dilutions for each application to be determined by the researcher