FLI1 Antibody [FLVI1-1]
| Code | Size | Price |
|---|
| PSI-34-039-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: FLVI1-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
Application Note:
Western blot: 1-2 ug/m;l
Flow Cytometry: 0.5-1ug/10^6 cells
IF: 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FLI1 antibody to be titered up or down for optimal performance.
Flow Cytometry: 0.5-1ug/10^6 cells
IF: 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FLI1 antibody to be titered up or down for optimal performance.
Background:
Friend leukemia integration 1 is a member of the ETS family of DNA binding transcription factors that is involved in cellular proliferation and tumorigenesis. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing's Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human partial recombinant protein (amino acids 107-216) was used as the immunogen for this FLI1 antibody.
NCBI Gene ID #:
2313
NCBI Official Name:
Fli-1 proto-oncogene, ETS transcription factor
NCBI Official Symbol:
FLI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cell Cycle,Obesity
Swissprot #:
Q01543
User NOte:
Optimal dilutions for each application to be determined by the researcher