Galectin 1 Antibody [GAL1/1831]
| Code | Size | Price |
|---|
| PSI-33-582-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, lambda
Antibody Clonality: Monoclonal
Antibody Clone: GAL1/1831
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Galectin-1, Gal-1, 14 kDa laminin-binding protein, HLBP14, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, LGALS1
Application Note:
ELISA: 2-4 ug/ml; order the BSA-free formulation for coating
IF: 1-2 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Titering of the Galectin 1 antibody may be required for optimal performance.
IF: 1-2 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Titering of the Galectin 1 antibody may be required for optimal performance.
Background:
Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein corresponding to amino acids 12-108 was used as the immunogen for the Galectin 1 antibody.
NCBI Gene ID #:
3956
NCBI Official Name:
lectin, galactoside-binding, soluble, 1
NCBI Official Symbol:
Lgals1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P09382
User NOte:
Optimal dilutions for each application to be determined by the researcher