GFAP Antibody [GFA12-3]
| Code | Size | Price |
|---|
| PSI-33-867-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: GFA12-3
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at 2-8C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Glial fibrillary acidic protein, GFAP, GFAP
Application Note:
Western blot: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the GFAP antibody may be required for optimal performance.
IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the GFAP antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
Glial fibrillary acidic protein is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [RefSeq]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Native Glial fibrillary acidic protein was used as the immunogen for this GFAP antibody.
NCBI Gene ID #:
2670
NCBI Official Name:
glial fibrillary acidic protein
NCBI Official Symbol:
GFAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P14136
User NOte:
Optimal dilutions for each application to be determined by the researcher