Recombinant Histone H1 Antibody [HH1/1784R]

ProSci
Product Code: PSI-34-090
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-34-090-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG, kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: HH1/1784R
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human pancreas and recombinant Histone H1 antibody (clone HH1/1784R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human pancreas and recombinant Histone H1 antibody (clone HH1/1784R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Further Information

Additional Names:
H1F0, H10, H1FV, MGC5241
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant full-length human protein was used as the immunogen for the rabbit monoclonal recombinant Histone H1 antibody.
NCBI Gene ID #:
3005
NCBI Official Name:
H1 histone family member 0
NCBI Official Symbol:
H1F0
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Cancer
Swissprot #:
P07305
User NOte:
Optimal dilutions for each application to be determined by the researcher