IgM Antibody [MuHC2]

Immunofluorescence: 0.5-1 ug/ml

Western blot: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. ?IgM antibodies account for approximately 5%-10% of all the antibody in the body.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Human Mu heavy chain was used as the immunogen for this IgM antibody.

3507

immunoglobulin heavy constant mu

IGHM

homo sapiens

Liquid

Protein G

Immunology

P01871

Optimal dilutions for each application to be determined by the researcher