CD8a Antibody [CT-8]

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ProSci
Product Code: PSI-99-212
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-99-212-0.5mg0.5mg£597.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: [CT-8]
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Pigeon
  • Turkey
Application: Flow Cytometry
Shipping:
blue ice
Storage:
Store vial at 2-8˚C

Images

1 / 4
Paraffin embedded chicken spleen tissue was stained with Mouse Anti-Chicken CD8-UNLB (Cat. No. 99-212; right) followed by Anti-Mouse IgG-HRP and DAB.
2 / 4
Paraffin embedded low pathogenic avian influenza virus infected lung section was stained with Mouse Anti-Chicken CD8-UNLB (Cat. No. 99-212) followed by a secondary antibody, DAB, and haematoxylin. Image from Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. Figure 4(a)
3 / 4
Paraffin embedded high pathogenic avian influenza virus infected lung section was stained with Mouse Anti-Chicken CD8-UNLB (Cat. No. 99-212) followed by a secondary antibody, DAB, and haematoxylin. Image from Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. Figure 4(c)
4 / 4
Frozen chicken ovarian tumor section was stained with Mouse Anti-Chicken CD8-UNLB (Cat. No. 99-212) followed by an HRP conjugated secondary antibody, DAB, and hematoxylin. Image from Bradaric MJ, Barua A, Penumatsa K, Yi Y, Edassery SL, Sharma S, et al. Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors. J Ovarian Res. 2011;4:4. Figure 7(d)
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Paraffin embedded chicken spleen tissue was stained with Mouse Anti-Chicken CD8-UNLB (Cat. No. 99-212; right) followed by Anti-Mouse IgG-HRP and DAB.
Paraffin embedded low pathogenic avian influenza virus infected lung section was stained with Mouse Anti-Chicken CD8-UNLB (Cat. No. 99-212) followed by a secondary antibody, DAB, and haematoxylin. Image from Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. Figure 4(a)
Paraffin embedded high pathogenic avian influenza virus infected lung section was stained with Mouse Anti-Chicken CD8-UNLB (Cat. No. 99-212) followed by a secondary antibody, DAB, and haematoxylin. Image from Rebel JM, Peeters B, Fijten H, Post J, Cornelissen J, Vervelde L. Highly pathogenic or low pathogenic avian influenza virus subtype H7N1 infection in chicken lungs: small differences in general acute responses. Vet Res. 2011;42:10. Figure 4(c)
Frozen chicken ovarian tumor section was stained with Mouse Anti-Chicken CD8-UNLB (Cat. No. 99-212) followed by an HRP conjugated secondary antibody, DAB, and hematoxylin. Image from Bradaric MJ, Barua A, Penumatsa K, Yi Y, Edassery SL, Sharma S, et al. Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors. J Ovarian Res. 2011;4:4. Figure 7(d)

Documents

Further Information

Application Note:
CD8a Antibody [CT-8] for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, and blocking assays.
Background:
In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two alpha chains and (ii) a heterodimer of an alpha chain and a beta chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCRgammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgammadelta CD8alphabeta cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody CT-8 recognizes the CD8alpha chain and has been shown to react to a polymorphic determinant in turkey.
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
0.5 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chicken thymocytes and Ig-negative blood leukocytes
NCBI Gene ID #:
403158
NCBI Official Name:
CD8a molecule
NCBI Official Symbol:
CD8A
NCBI Organism:
Gallus gallus
Physical State:
Liquid
Research Area:
Cancer,Immunology,Signal Transduction
SPECIFICITY:
CD8alpha
User NOte:
Optimal dilutions for each application to be determined by the researcher.