CD8a Antibody [CT-8] (FITC)

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ProSci
Product Code: PSI-99-213
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-99-213-0.5mg0.5mg£729.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: [CT-8]
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Pigeon
  • Turkey
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunoprecipitation (IP)
Shipping:
blue ice
Storage:
Store vial at 2-8˚C

Images

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Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken CD8-FITC (Cat. No. 99-213) and Mouse Anti-Chicken CD4-PE .
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Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken CD8-FITC (Cat. No. 99-213) and Mouse Anti-Chicken CD4-PE .

Documents

Further Information

Application Note:
CD8a Antibody [CT-8] for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
Background:
In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two alpha chains and (ii) a heterodimer of an alpha chain and a beta chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCRgammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgammadelta CD8alphabeta cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody CT-8 recognizes the CD8alpha chain and has been shown to react to a polymorphic determinant in turkey.
Buffer:
Supplied in PBS/NaN3
Concentration:
0.5 mg/mL
Conjugate:
FITC
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chicken thymocytes and Ig-negative blood leukocytes
NCBI Gene ID #:
403158
NCBI Official Name:
CD8a molecule
NCBI Official Symbol:
CD8A
NCBI Organism:
Gallus gallus
Physical State:
Liquid
Research Area:
Cancer,Immunology,Signal Transduction
SPECIFICITY:
CD8alpha
User NOte:
Optimal dilutions for each application to be determined by the researcher.