CD8beta Antibody [EP42]

CD8beta Antibody [EP42] for use in flow cytometry, immunohistochemistry, and immunoprecipitation assays.

In the chicken, the CD8 molecule is present in two forms - (i) a homodimer of two alpha chains and (ii) a heterodimer of an alpha chain and a beta chain. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR-gammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCR-gammadelta CD8alphabeta cells in the chicken that is not found in rodents. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. The monoclonal antibody EP42 recognizes the CD8beta chain.

Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

0.5 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Chicken splenocytes

396175

CD8b molecule

CD8B

Gallus gallus

Liquid

Cancer,Immunology

CD8beta

Optimal dilutions for each application to be determined by the researcher.