CD8a Antibody [53-6.7]
| Code | Size | Price |
|---|
| PSI-98-620-0.5mg | 0.5mg | £541.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: [53-6.7]
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunoprecipitation (IP)
Shipping:
blue ice
Storage:
Store vial at -20˚C
Documents
Further Information
Additional Names:
Ly-2, Lyt-2, Ly-35, Ly-B, T8
Application Note:
CD8a Antibody [53-6.7] for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, depletion, and blocking assays.
Background:
In the mouse, CD8 exists in two forms ? (i) a CD8 heterodimer composed of an alpha chain (CD8alpha) and a beta chain (CD8beta); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ?suppressor/cytotoxic? subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8alpha without CD8beta. It has been suggested that CD8+beta- T cells mature extrathymically, while development of the CD8alpha+beta+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8alpha+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Buffer:
Supplied in PBS
Concentration:
0.5 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Spleen cells or thymocyte membranes
NCBI Gene ID #:
12525
NCBI Official Name:
CD8 antigen, alpha chain
NCBI Official Symbol:
Cd8a
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
P01731
Research Area:
Immunology
SPECIFICITY:
CD8alpha
Swissprot #:
P01731
User NOte:
Optimal dilutions for each application to be determined by the researcher.