GCDFP-15 Antibody [PIP/1571]

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ProSci
Product Code: PSI-33-648
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-648-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PIP/1571
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.
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IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.

Documents

Further Information

Additional Names:
Prolactin-inducible protein, Gross cystic disease fluid protein 15, GCDFP-15, Prolactin-induced protein, Secretory actin-binding protein, SABP, gp17, PIP, GCDFP15, GPIP4
Application Note:
IF: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

WB: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

Titering of the PIP antibody may be required for optimal performance.
Background:
It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15), also called Prolactin Inducible Protein, or PIP. It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. PIP is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein corresponding to amino acids 41-146 was used as the immunogen for the PIP antibody.
NCBI Gene ID #:
5304
NCBI Official Name:
prolactin-induced protein
NCBI Official Symbol:
PIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Signal Transduction
Swissprot #:
P12273
User NOte:
Optimal dilutions for each application to be determined by the researcher