TIMP2 Antibody [SPM356]

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ProSci
Product Code: PSI-33-734
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-734-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM356
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human placenta with TIMP2 antibody (clone SPM356). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human placenta with TIMP2 antibody (clone SPM356). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Additional Names:
TIMP2, Metalloproteinase inhibitor 2, TIMP-2, CSC-21K
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

WB: 0.5-1 ug/ml

IF: 1-2 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.
Background:
It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2). It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP9 or gelatinase B). TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
An amino acid sequence from the N-terminal region of the human protein was used as the immunogen for this TIMP2 antibody.
NCBI Gene ID #:
7077
NCBI Official Name:
TIMP metallopeptidase inhibitor 2
NCBI Official Symbol:
TIMP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P16035
User NOte:
Optimal dilutions for each application to be determined by the researcher