Gamma Catenin Antibody [11E4.]

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ProSci
Product Code: PSI-33-585
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-585-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 11E4.
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Western blot testing of human 1) HT20, 2) A549, 3) HEK293 and 4) A431 cell lysate with Plakoglobin antibody (clone 11E4). Predicted molecular weight: 80-87 kDa.
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Western blot testing of human 1) HT20, 2) A549, 3) HEK293 and 4) A431 cell lysate with Plakoglobin antibody (clone 11E4). Predicted molecular weight: 80-87 kDa.

Documents

Further Information

Additional Names:
Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3, JUP, CTNNG, DP3
Application Note:
Immunofluorescence: 1-2 ug/ml

WB: 0.5-1 ug/ml

Immunoprecipitation: suitable

Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Background:
It recognizes a protein of 80-87kDa, identified as gamma-Catenin or Plakoglobin. The catenins (alpha, beta, gamma and δ) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. alpha T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. alpha T-catenin binds to alpha E-catenin as well as to beta-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for alpha-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant full length human gamma Catenin fused to maltose binding protein was used as the immunogen for the Plakoglobin antibody. The epitope has been found to be in the N-terminal region.
NCBI Gene ID #:
3728
NCBI Official Name:
junction plakoglobin
NCBI Official Symbol:
JUP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P14923
User NOte:
Optimal dilutions for each application to be determined by the researcher