Calnexin Antibody [CANX/1541]

WB: 0.5-1 ug/ml

Flow Cytometry: 0.5-1ug/10^6 cells

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT

Titering of the Calnexin antibody may be required for optimal performance.

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

A partial human recombinant protein was used as the immunogen for the Calnexin antibody.

Liquid

Protein G affinity chromatography

Neuroscience

Does not react with rat

Optimal dilutions for each application to be determined by the researcher