Biotin Antibody [SPM375]
| Code | Size | Price |
|---|
| PSI-34-197-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: SPM375
Regulatory Status: RUO
Applications:
- Functional Study
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Application Note:
Confocal Microscopy
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
In situ Nucleic Acid Hybridization
Western: blot 1-3 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
In situ Nucleic Acid Hybridization
Western: blot 1-3 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.
1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Background:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
Not Applicable
User NOte:
Optimal dilutions for each application to be determined by the researcher