Cytokeratin 15 Antibody [KRT15/1699]

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ProSci
Product Code: PSI-33-678
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-678-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: KRT15/1699
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human basal cell carcinoma with Cytokeratin 10 antibody (clone KRT15/1699). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human basal cell carcinoma with Cytokeratin 10 antibody (clone KRT15/1699). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Documents

Further Information

Additional Names:
KRT15, CK15, K15, K1CO
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

WB: 0.5-1 ug/ml

Immunohistochemistry (FFPE & Frozen): 0.5-1 ug/ml for 30 min at RT

Optimal dilution of the Cytokeratin 15 antibody should be determined by the researcher.
Background:
Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human KRT15 protein was used as the immunogen for the Cytokeratin 15 antibody.
NCBI Gene ID #:
3866
NCBI Official Name:
keratin 15
NCBI Official Symbol:
KRT15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Transcription
Swissprot #:
P19012
User NOte:
Optimal dilutions for each application to be determined by the researcher