KRT8 Antibody [K8.8]
| Code | Size | Price |
|---|
| PSI-33-166-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: K8.8
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
KRT8, CARD2, CK8, CYK8, K2C8, K8, KO, CK-8
Application Note:
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Differences in protocols and secondaries may require the KRT8 antibody to be titered for optimal performance.
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Differences in protocols and secondaries may require the KRT8 antibody to be titered for optimal performance.
1. FFPE staining requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
KRT8, also called Cytokeratin 8, is a low molecular weight keratin of approximately 50 kDa. It participates in cellular differentiation and signal transduction, protects against apoptosis, stress and injury, and helps maintain cellular structural integrity. KRT8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human carcinoma keratin was used as the immunogen for this KRT8 antibody.
NCBI Gene ID #:
3856
NCBI Official Name:
keratin 8
NCBI Official Symbol:
KRT8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
SPECIFICITY:
Does not react with rat
Swissprot #:
P05787
User NOte:
Optimal dilutions for each application to be determined by the researcher