Hepatocyte Specific Antigen Antibody [HSA133]

ProSci
Product Code: PSI-33-566
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-566-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: HSA133
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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SDS-PAGE Analysis of Purified, BSA-Free Hepatocyte Specific Antigen Antibody (clone HSA133). Confirmation of Integrity and Purity of the Antibody.

SDS-PAGE Analysis of Purified, BSA-Free Hepatocyte Specific Antigen Antibody (clone HSA133). Confirmation of Integrity and Purity of the Antibody.

Further Information

Application Note:
Immunofluorescence: 0.5-1 ug/ml

Immunocytochemistry (Acetone-fixed cells): 0.5-1 ug/ml for 30 minutes at RT

The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.
Background:
mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
Not Known
User NOte:
Optimal dilutions for each application to be determined by the researcher