FTL1 Antibody [FTL/1386]

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ProSci
Product Code: PSI-33-711
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-711-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: FTL/1386
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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SDS-PAGE Analysis of Purified, BSA-Free Ferritin Light Chain Antibody (clone FTL/1386). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free Ferritin Light Chain Antibody (clone FTL/1386). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
FTL, MGC71996, LFTD, NBIA3
Application Note:
Western blot: 0.1-0.2 ug/ml

Flow Cytometry: 0.1-0.2ug/10^6 cells

IF: 0.1-0.2 ug/ml

IHC (FFPE): 0.1-0.2 ug/ml for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin Light Chain antibody to be titered up or down for optimal performance.
Background:
Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin Light Chain antibody.
NCBI Gene ID #:
2512
NCBI Official Name:
ferritin, light polypeptide
NCBI Official Symbol:
FTL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Obesity, Neuroscience, Signal Transduction
Swissprot #:
P02792
User NOte:
Optimal dilutions for each application to be determined by the researcher